Twitch is one of the toys from Sunnyside Daycare that appears in Toy Story 3. He is an "insectaloid warrior" action figure with a bug's head. He has red eyes with ferocious chomping mandibles, wings, and two muscular arms. He is voiced by John Cygan.

He is one of the strongest toys serving Lots-O'-Huggin' Bear, and helps apprehend Andy's toys, and keeps a search light working in the playground. Twitch stands over five inches tall with more than 15 points of articulation. He also carries a signature magical battle staff and has removable chest armor. Twitch was also one of Lotso's henchmen. He has shown to be laidback but rough. As with his cohorts, Twitch is convinced of Lotso's deception by Woody and Ken, and is shocked when Lotso mistreats Big Baby and pushes him aside abusively.

In the end credits, he is shown happily taking a turn with Chunk as they endure a rough playtime with the abusive toddlers in the Caterpillar Room.